A single medium and sequential media are equally effective for the culture of human embryos to the blastocyst stage: a pilot study

ثبت نشده
چکیده

Ovulation Induction and Oocyte Recovery Ovarian hyperstimulation was achieved using GnRH analogue (nafarelin acetate, Synarel®, Pfizer) along with human recombinant FSH (r-hFSH: Gonal-F®, MerckSerono) in a long down-regulation protocol. Starting gonadotrophin dose varied from 150-300 IU and was based on female age, body mass index, ovarian volume, basal FSH levels and the number of preantral follicles. Human chorionic gonadotrophin (hCG; Ovidrel®, MerckSerono) was administered when at least one follicle reached 18 mm in diameter on ultrasound scan. Oocytes were collected 34-36 hours after hCG administration under transvaginal ultrasound guidance.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

P-63: Comparison of The Efficacy of Two Culture Media (Handmade vs. Commercial) on In Vitro Development of Vitrified and Non-Vitrified Mouse Embryos

Background: While assisted reproductive techniques (ARTs) are considered to be safe, many concerns exist about the safety of these techniques. Two important and indispensable parts of these techniques are in vitro culture and vitrification. While much progress has been made in developing culture conditions, it remains suboptimal. In order to investigate the effect of culture medium on in vitro ...

متن کامل

O-23: Transfer of Blastocysts Derived from Frozen Thawed Cleavage Stage Embryos Improved Ongoing Pregnancy

Background: The aim of our study was to compare the resulting from transfer of embryos that cryopereserved in cleavage stage after thawing and transfer of embryos after thawing and culture in sequentional media until blastocyst formation. Materials and Methods: In this prospective clinical study, we have evaluated 128 cycles in women undergone in vitro fertilization (IVF) or intracytoplasmic sp...

متن کامل

Highly Efficient In Vitro Production of Bovine Blastocyst in Cell-Free Sequential Synthetic Oviductal Fluid vs. TCM199 Vero Cell Co-Culture System

Background The aim of this study was to establish a cell-free sequential culture system that can support high levels of in vitro embryo development and blastocyst formation from bovine zygotes. To this end, this investigation was carried out to evaluate the effects of glucose, serum and EDTA on bovine zygote in vitro development. MaterialsAndMethods Bovine presumptive zygotes were derived from ...

متن کامل

Effect of LH Treated Ovine Oviductal Epithelial Cell Co-Culture System on Murine Pre-Embryo Development

Background This study was designed to develop a new co-culture system, assess the effect of luteinizing hormone (LH) using sequential media to promote development and increase the quality of 2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages. MaterialsAndMethods Monolayers for co-culture were prepared from ovine oviduct epithelial cells (OOEC) in DMEM/F12 medium a...

متن کامل

Differential Effect of Medium on the Ratio of ICM/TE of Bovine Embryos in a Co-culture System

Background This study was undertaken to investigate the efficiency of two different embryo somatic cell co-culture conditions tissue culture medium 199 (TCM199)–vero cells and Menezo B2 (B2)-vero cells for the in vitro developmental quantity and quality of bovine embryos. MaterialsAndMethods Bovine oocytes were allowed to mature and subsequently undergo fertilization in vitro. Their presumptive...

متن کامل

Assessment of Epidermal Growth Factor (EGF) Effects on Development of Vitrified Mouse Morulae to the Blastocyst Stage

Many investigators are interested in finding the new cultural systems that can support the in vitro development of pre-implantation embryos better. Previous studies suggested that growth factors such as epidermal growth factor (EGF) are important in pre-implantation embryo development and implantation process. On the other hand, it is very important to support post thaw development of frozen em...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2009